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Mutations in the fatty acid elongation 1 gene are associated with a loss of β‐ketoacyl‐CoA synthase activity in low erucic acid rapeseed
Author(s) -
Roscoe T.J,
Lessire R,
Puyaubert J,
Renard M,
Delseny M
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02243-8
Subject(s) - erucic acid , biochemistry , microsome , rapeseed , fatty acid , acyl carrier protein , biology , malonyl coa , atp synthase , acyl coa , fatty acid synthase , gene , microbiology and biotechnology , enzyme , biosynthesis , beta oxidation , botany
Low erucic acid rapeseed (LEAR) is characterised by a near absence of very long chain fatty acids in the seed oil which has been correlated with a lack of acyl‐CoA elongation activity. Here we show that the absence of acyl‐CoA and ATP‐dependent elongation activities in microsomes isolated from LEAR embryos is associated with an absence of β‐ketoacyl‐CoA synthase activity encoded by the Bn‐ fatty acid elongation 1 ( FAE1 ) genes. Size exclusion chromatography of solubilised microsomes revealed the presence of a high molecular mass acyl‐CoA elongase complex in high erucic acid rapeseed which was absent in microsomes isolated from LEAR seeds. Although transcripts for the Bn‐ FAE1 genes were detected in LEAR embryos, immunoblots using antisera raised against the β‐ketoacyl‐CoA synthase indicated an absence of this protein. Comparison of the deduced amino acid sequences of immature embryo cDNAs reveals that LEAR alleles of Bn‐ FAE1 encode variant β‐ketoacyl‐CoA synthase proteins.