Premium
Hematopoietic cell‐specific adapter proteins, SLP‐76 and BLNK, effectively activate NF‐AT as well as NF‐κB by Syk and Tec PTKs in non‐lymphoid cell lines
Author(s) -
Yamamoto Tetsuya,
Matsuda Tadashi,
Junicho Akira,
Kishi Hiroyuki,
Yoshimura Akihiko,
Muraguchi Atsushi
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02208-6
Subject(s) - syk , microbiology and biotechnology , biology , signal transducing adaptor protein , t cell receptor , b cell , signal transduction , t cell , jurkat cells , tyrosine kinase , immunology , immune system , antibody
To investigate the roles of various hematopoietic cell‐specific adapter proteins in T cell receptor (TCR)‐signaling leading to nuclear factor of activated T cell (NF‐AT) and nuclear factor of κB (NF‐κB) activation, we reconstituted TCR‐signaling with CD8/ζ, various protein tyrosine kinases (PTKs), and adapter proteins in a non‐lymphoid cell line, 293T. We show that SLP‐76 and BLNK, but not LAT, effectively co‐operated with Syk and Tec family PTKs to activate NF‐AT and NF‐κB. We also show that Tec family PTKs enhanced endogenous phospholipase C (PLC)‐γ1 phosphorylation induced by CD8/ζ and Syk in 293T cells. These results imply that PLC‐γ1 may play a critical role in a hematopoietic cell‐specific adapter protein‐mediated NF‐AT and NF‐κB activation in a non‐lymphoid cell.