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Differential skeletal muscle expression of myostatin across teleost species, and the isolation of multiple myostatin isoforms
Author(s) -
Roberts Steven B,
Goetz Frederick W
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02196-2
Subject(s) - myostatin , gene isoform , biology , hindbrain , complementary dna , medicine , endocrinology , vertebrate , skeletal muscle , gene , central nervous system , genetics
Two myostatin (MSTN) isoforms were isolated from brook trout with 92% identity in corresponding regions at the nucleotide level. One isoform was isolated from muscle and brain and the second from ovarian tissue. To our knowledge this is the first time two MSTN isoforms have been isolated from a given vertebrate species. Within the brain, MSTN transcripts were localized to the optic lobes, hindbrain, and hypothalamus. In the trout ovary, MSTN transcripts were upregulated at ovulation in several females. MSTN cDNA fragments were also isolated from several other fish species and differential expression of MSTN among muscle fiber types was observed.