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A novel patatin‐like gene stimulated by drought stress encodes a galactolipid acyl hydrolase
Author(s) -
Matos Ana Rita,
d'Arcy-Lameta Agnès,
França Marcel,
Pêtres Stéphane,
Edelman Léna,
Kader Jean-Claude,
Zuily-Fodil Yasmine,
Pham-Thi Anh Thu
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02194-9
Subject(s) - galactolipid , complementary dna , biology , gene , vigna , biochemistry , hydrolase , gene expression , microbiology and biotechnology , enzyme , botany , chloroplast
A cDNA ( Vupat1 ) encoding a predicted 43 kDa protein was isolated from drought‐stressed cowpea ( Vigna unguiculata ) leaves. It has homology with patatin, a potato tuber storage protein with lipolytic acyl hydrolase activity. The recombinant protein VUPAT1 expressed in the baculovirus system displays preferentially galactolipid acyl hydrolase activity. Phospholipids are very slowly hydrolyzed and apparently triacylglycerols are not deacylated. Vupat1 promoter contains putative drought‐inducible sequences. Northern blots showed that gene expression is stimulated by drought stress and is more pronounced in a drought‐sensitive cultivar than in a drought‐tolerant one. An involvement in drought‐induced galactolipid degradation is proposed for VUPAT1.