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Involvement of histone H4 gene transcription factor 1 in downregulation of vimentin gene expression during skeletal muscle differentiation
Author(s) -
Kryszke Marie-Hélène,
Moura-Neto Vivaldo,
Lilienbaum Alain,
Paulin Denise,
Auclair Christian
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02142-1
Subject(s) - myogenesis , vimentin , desmin , downregulation and upregulation , biology , transcription factor , microbiology and biotechnology , gene expression , intermediate filament , mef2 , myocyte , gene , genetics , cytoskeleton , enhancer , immunology , cell , immunohistochemistry
Upon in vitro myogenesis, the intermediate filament protein vimentin is replaced by desmin, the switch in gene expression occurring essentially at the transcriptional level. Trying to elucidate the molecular mechanisms of this genetic control, we show here that the vimentin promoter is specifically recognized and activated by a protein most probably identical to H4TF‐1, and that this factor is present in proliferating myoblasts but disappears upon fusion of these cells into multinucleated myotubes. Our results suggest that H4TF‐1 is a differentiation stage‐specific factor involved in the downregulation of vimentin gene expression during myogenesis.