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Syk protein‐tyrosine kinase is involved in neuron‐like differentiation of embryonal carcinoma P19 cells
Author(s) -
Tsujimura Toshiaki,
Yanagi Shigeru,
Inatome Ryoko,
Takano Tomoko,
Ishihara Itsuko,
Mitsui Norihiro,
Takahashi Shusuke,
Yamamura Hirohei
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02097-x
Subject(s) - syk , p19 cell , neurite , microbiology and biotechnology , mapk/erk pathway , neuron , signal transduction , cancer research , cellular differentiation , biology , tyrosine kinase , chemistry , neuroscience , biochemistry , adult stem cell , gene , in vitro
Syk has been implicated in activated immunoreceptors to downstream signaling events in hematopoietic cells. Here we report that Syk is expressed in neuron‐like cells and involved in neuron‐like differentiation of embryonal carcinoma P19 cells. Immunoblot, RT‐PCR, and Northern analysis indicated that Syk is expressed in mouse brain, PC12 and P19 cells. In addition, Syk was found to be tyrosine phosphorylated during neuron‐like differentiation of P19 cells. Furthermore, adenovirus‐mediated overexpression of Syk induced supernumerary neurite formation and extracellular signal‐regulated kinase (ERK) activation in P19 cells. These results suggest that Syk plays an important role in signaling steps leading to ERK activation in P19 cells.