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An Arabidopsis T‐DNA mutant affected in Nrt2 genes is impaired in nitrate uptake
Author(s) -
Filleur Sophie,
Dorbe Marie-France,
Cerezo Miguel,
Orsel Mathilde,
Granier Fabienne,
Gojon Alain,
Daniel-Vedele Françoise
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02096-8
Subject(s) - mutant , arabidopsis , gene , arabidopsis thaliana , biology , heterologous , mutation , genetics , function (biology) , heterologous expression , dna , wild type , gene expression , microbiology and biotechnology , recombinant dna
Expression analyses of Nrt2 plant genes have shown a strict correlation with root nitrate influx mediated by the high‐affinity transport system (HATS). The precise assignment of NRT2 protein function has not yet been possible due to the absence of heterologous expression studies as well as loss of function mutants in higher plants. Using a reverse genetic approach, we isolated an Arabidopsis thaliana knock‐out mutant where the T‐DNA insertion led to the complete deletion of the AtNrt2.1 gene together with the deletion of the 3′ region of the AtNrt2.2 gene. This mutant is impaired in the HATS, without being modified in the low‐affinity system. Moreover, the de‐regulated expression of a Nicotiana plumbaginifolia Nrt2 gene restored the mutant nitrate influx to that of the wild‐type. These results demonstrate that plant NRT2 proteins do have a role in HATS.