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Identification of two proteins, S14 and UIP1, that interact with UCH37
Author(s) -
Li Tianwei,
Duan Wei,
Yang Hongyuan,
Lee Mui-Khin,
Bte Mustafa Fatimah,
Lee Boon-Heng,
Teo Tian-Seng
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02436-4
Subject(s) - yeast , two hybrid screening , in vitro , immunoprecipitation , microbiology and biotechnology , protein subunit , plasma protein binding , protein–protein interaction , biology , biochemistry , chemistry , gene
By the use of the yeast two‐hybrid screen we have identified two proteins that interacted with UCH37: S14, which is a subunit of PA700 and a novel protein, UIP1 (UCH37 interacting protein 1). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in vivo co‐immunoprecipitation analysis. The C‐terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two‐hybrid assay and the in vitro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro.