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High‐yield expression of a viral peptide vaccine in transgenic plants
Author(s) -
Gil Félix,
Brun Alejandro,
Wigdorovitz Andrés,
Catalá Rafael,
Martı́nez-Torrecuadrada Jorge L.,
Casal Ignacio,
Salinas Julio,
Borca Manuel V.,
Escribano José M.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02405-4
Subject(s) - immunogenicity , transgene , genetically modified crops , biology , fusion protein , recombinant dna , virology , peptide , epitope , chimeric gene , peptide vaccine , microbiology and biotechnology , gene , gene expression , antigen , biochemistry , immunology
A high‐yield production of a peptide vaccine in transgenic plants is described here. A 21‐mer peptide, which confers protection to dogs against challenge with virulent canine parvovirus, has been expressed in transgenic plants as an amino‐terminal translational fusion with the GUS gene. Transformants were selected on the basis of their GUS activities, showing expression levels of the recombinant protein up to 3% of the total leaf soluble protein, a production yield comparable to that obtained with the same epitope expressed by chimeric plant viruses. The immunogenicity of the plant‐derived peptide was demonstrated in mice immunized either intraperitoneally or orally with transgenic plant extracts, providing the suitability of the GUS fusions approach for low‐cost production of peptide vaccines.