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Expression of telomerase inhibits hydroxyl radical‐induced apoptosis in normal telomerase negative human lung fibroblasts
Author(s) -
Ren Jian-Guo,
Xia Hui-Li,
Tian Yan-Mei,
Just Tom,
Cai Guo-Ping,
Dai Yao-Ren
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02397-8
Subject(s) - telomerase , telomerase reverse transcriptase , telomere , apoptosis , microbiology and biotechnology , transfection , chemistry , protein subunit , cancer research , biology , cell culture , gene , biochemistry , genetics
In tumor cells telomerase activity is associated with resistance to apoptosis and the introduction of the human telomerase reverse transcriptase (hTERT) subunit into normal human cells is associated with life span extension of the cells. To determine the role of telomerase in regulating apoptosis, telomerase negative human embryo lung fibroblasts were transfected with the hTERT gene. Unlike the control fibroblasts, the telomerase‐expressing cells had elongated telomeres and were resistant to apoptosis induced by hydroxyl radicals. The results indicate that expression of telomerase and, thus, the maintenance of telomere length in normal human somatic cells caused resistance to not only cellular senescence but also apoptosis. Moreover, we found that hydroxyl radical‐induced apoptosis in telomerase‐expressing and control fibroblasts was caspase‐3 independent. These findings have revealed a new type of interrelation between telomerase and caspase‐3, which may indicate that in this case the expressed telomerase may inhibit apoptosis at a site not related to the caspase‐3 cascade.