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Identification of mouse Jun dimerization protein 2 as a novel repressor of ATF‐2
Author(s) -
Jin Chunyuan,
Ugai Hideyo,
Song Jun,
Murata Takehide,
Nili Feuerstein,
Sun Kailai,
Horikoshi Masami,
Yokoyama Kazunari K.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02387-5
Subject(s) - leucine zipper , activating transcription factor , repressor , atf3 , bzip domain , transcription factor , activating transcription factor 2 , creb1 , microbiology and biotechnology , gatad2b , dna binding domain , biology , yy1 , gene , dna binding protein , complementary dna , basic helix loop helix leucine zipper transcription factors , response element , chemistry , biochemistry , gene expression , creb , promoter
A mouse cDNA that encodes a DNA‐binding protein was identified by yeast two‐hybrid screening, using activating transcription factor‐2 (ATF‐2) as the bait. The protein contained a bZIP (basic amino acid‐leucine zipper region) domain and its amino acid sequence was almost identical to that of rat Jun dimerization protein 2 (JDP2). Mouse JDP2 interacted with ATF‐2 both in vitro and in vivo via its bZIP domain. It was encoded by a single gene and various transcripts were expressed in all tested tissues of adult mice, as well as in embryos, albeit at different levels in various tissues. Furthermore, mouse JDP2 bound to the cAMP‐response element (CRE) as a homodimer or as a heterodimer with ATF‐2, and repressed CRE‐dependent transcription that was mediated by ATF‐2. JDP2 was identified as a novel repressor protein that affects ATF‐2‐mediated transcription.