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Prolactin activation of IRF‐1 transcription involves changes in histone acetylation
Author(s) -
Book McAlexander Melissa,
Yu-Lee Li-yuan
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02385-1
Subject(s) - chromatin immunoprecipitation , histone h4 , acetylation , histone , transcription factor , prolactin , transcription (linguistics) , chromatin , microbiology and biotechnology , chemistry , hdac8 , promoter , biology , histone h2a , gene expression , gene , biochemistry , hormone , linguistics , philosophy
In response to prolactin (PRL) signaling, transcription of the interferon regulatory factor‐1 gene (IRF‐1) is rapidly induced during early G 1 , declines in mid G 1 , and rises again over the G 1 /S transition phase of the cell cycle in Nb2 T cells. Using chromatin immunoprecipitation assays, we show that histone H4 acetylation increases over a 1.7 kb region of the IRF‐1 promoter in early G 1 and again at the G 1 /S transition in response to PRL stimulation. These results demonstrate a correlation between histone H4 hyperacetylation at the IRF‐1 promoter and biphasic transcription of IRF‐1 in response to PRL signaling in vivo.