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On a revised mechanism of side product formation in the lignin peroxidase catalyzed oxidation of veratryl alcohol
Author(s) -
ten Have Rimko,
Franssen Maurice C.R.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02379-6
Subject(s) - chemistry , peroxidase , lignin , alcohol , lignin peroxidase , catalysis , reaction mechanism , medicinal chemistry , mechanism (biology) , organic chemistry , side chain , photochemistry , stereochemistry , enzyme , philosophy , polymer , epistemology
The O 2 ‐dependent formation of side products during the oxidation of veratryl alcohol (VA) by lignin peroxidase has previously been proposed to start with the attack of H 2 O on the VA radical cation (VA + ). This initial reaction is unlikely since it would also lead to side product formation in the absence of O 2 , which is not the case. In the current mechanism VA + reacts first with O 2 , whereafter H 2 O attacks. Furthermore, this paper describes an alternative explanation for the inhibitory effect of Mn 2+ on VA side product formation. It is proposed that Mn 2+ reduces reactive intermediates back to VA.