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Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes
Author(s) -
Canfield Ann E.,
Doherty Mary Jo,
Kelly Victoria,
Newman Bill,
Farrington Claire,
Grant Michael E.,
Boot-Handford Raymond P.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02363-2
Subject(s) - pericyte , matrix gla protein , complementary dna , calcification , matrix (chemical analysis) , cdna library , microbiology and biotechnology , immunohistochemistry , biology , extracellular matrix , chemistry , pathology , gene , biochemistry , ectopic calcification , endothelial stem cell , immunology , medicine , chromatography , in vitro
PCR‐based subtractive hybridisation was used to identify genes up‐regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification.