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Ca 2+ sensors of L‐type Ca 2+ channel
Author(s) -
Romanin Christoph,
Gamsjaeger Roland,
Kahr Heike,
Schaufler Daniela,
Carlson Olga,
Abernethy Darrell R.,
Soldatov Nikolai M.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02361-9
Subject(s) - calmodulin , chemistry , protein subunit , binding site , biophysics , calcium , crystallography , stereochemistry , biochemistry , biology , gene , organic chemistry
Ca 2+ ‐induced inactivation of L‐type Ca 2+ is differentially mediated by two C‐terminal motifs of the α 1C subunit, L (1572–1587) and K (1599–1651) implicated for calmodulin binding. We found that motif L is composed of a highly selective Ca 2+ sensor and an adjacent Ca 2+ ‐independent tethering site for calmodulin. The Ca 2+ sensor contributes to higher Ca 2+ sensitivity of the motif L complex with calmodulin. Since only combined mutation of both sites removes Ca 2+ ‐dependent current decay, the two‐site modulation by Ca 2+ and calmodulin may underlie Ca 2+ ‐induced inactivation of the channel.