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Potential role of group X secretory phospholipase A 2 in cyclooxygenase‐2‐dependent PGE 2 formation during colon tumorigenesis
Author(s) -
Morioka Yasuhide,
Ikeda Minoru,
Saiga Akihiko,
Fujii Noriko,
Ishimoto Yoshikazu,
Arita Hitoshi,
Hanasaki Kohji
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02350-4
Subject(s) - arachidonic acid , cyclooxygenase , carcinogenesis , phospholipase a2 , eicosanoid , prostaglandin , phospholipase a , prostaglandin e2 , phospholipase , adenocarcinoma , prostaglandin e , enzyme , chemistry , biochemistry , medicine , biology , endocrinology , cancer research , gene , cancer
Although the cyclooxygenase‐2 (COX‐2) pathway of the arachidonic acid cascade has been suggested to play an important role in colon carcinogenesis, there is little information concerning the identity of phospholipase A 2 (PLA 2 ) involved in the arachidonic acid release in colon tumors. Here, we compared the potencies of three types of secretory PLA 2 s (group IB, IIA and X sPLA 2 s) for the arachidonic acid release from cultured human colon adenocarcinoma cells, and found that group X sPLA 2 has the most powerful potency in the release of arachidonic acid leading to COX‐2‐dependent prostaglandin E 2 (PGE 2 ) formation. Furthermore, immunohistological analysis revealed the elevated expression of group X sPLA 2 in human colon adenocarcinoma neoplastic cells in concert with augmented expression of COX‐2. These findings suggest a critical role of group X sPLA 2 in the PGE 2 biosynthesis during colon tumorigenesis.