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Conserved gene structure and transcription factor sites in the human and mouse deoxycytidine kinase genes 1
Author(s) -
Johansson Magnus,
Norda Ameli,
Karlsson Anna
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02347-4
Subject(s) - deoxycytidine kinase , biology , microbiology and biotechnology , gene , transcription (linguistics) , transcription factor , enhancer , genetics , deoxycytidine , cancer , linguistics , philosophy , gemcitabine
Deoxycytidine kinase (dCK) phosphorylates several anti‐cancer and anti‐viral nucleoside analogs. The enzyme is predominantly expressed in lymphoid tissues regulated by an unknown mechanism. We have cloned and sequenced the 20 kbp mouse dCK gene and ≈1.7 kbp of the 5′ flanking regions of both the human and mouse dCK genes. Five major inter‐species conserved motifs were identified in the 5′ region including the transcription initiation region, an SP1 site and two closely located putative octamer transcription factor sites. Luciferase reporter experiments showed that the human dCK 5′ region efficiently initiated transcription but no tissue regulatory element could be identified.