Premium
Intracellular trehalose improves osmotolerance but not desiccation tolerance in mammalian cells
Author(s) -
Garcı́a de Castro Arcadio,
Tunnacliffe Alan
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02340-1
Subject(s) - trehalose , desiccation , desiccation tolerance , cryptobiosis , osmoprotectant , intracellular , biology , osmotic shock , dehydration , gene , biochemistry , microbiology and biotechnology , botany , proline , amino acid
Trehalose has been shown to play a role in osmotolerance or desiccation tolerance in some microorganisms, anhydrobiotic invertebrates and resurrection plants. To test whether trehalose could improve stress responses of higher eukaryotes, a mouse cell line was genetically engineered to express bacterial trehalose synthase genes. We report that the resulting levels of intracellular trehalose (∼80 mM) are able to confer increased resistance to the partial dehydration resulting from hypertonic stress, but do not enable survival of complete desiccation due to air drying.