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Modulation of WHSC 2 expression in human endothelial cells
Author(s) -
Mariotti M,
Manganini M,
Maier J.A.M
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02335-8
Subject(s) - biology , transfection , repressor , gene , microbiology and biotechnology , in vitro , basic helix loop helix , 3t3 cells , gene expression , genetics , transcription factor , dna binding protein
WHSC 2, a novel gene recently isolated within the critical region of Wolf–Hirschhorn syndrome, is expressed in endothelial cells. WHSC 2 is downregulated by HIV‐1 Tat, whereas it is not modulated by angiogenic and pro‐differentiative molecules. WHSC 2 encodes a basic polypeptide of 528 amino acids. The in vitro translated protein shows a molecular weight of 57 kDa. WHSC2 has two nuclear translocation sequences which actively mediate its transport to the nucleus, as shown in whsc2‐GFP‐transfected NIH‐3T3. We also found a helix‐loop‐helix (HLH) motif in region 130–185. Since members of the HLH family control differentiation and cell cycle progression, we hypothesize that WHSC2 may function as a transcriptional repressor.