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Occurrence of ceramides and neutral glycolipids with unusual long‐chain base composition in purified rat liver mitochondria
Author(s) -
Ardail D,
Popa I,
Alcantara K,
Pons A,
Zanetta J.P,
Louisot P,
Thomas L,
Portoukalian J
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02332-2
Subject(s) - lactosylceramide , glycolipid , ceramide , sphingosine , membrane , sphingolipid , biochemistry , mitochondrion , chemistry , sphingomyelin , inner mitochondrial membrane , inner membrane , glycosphingolipid , biology , chromatography , apoptosis , receptor
The free ceramide content of rat liver mitochondria was found to be 1.7 nmol/mg protein and outer membranes contained a three‐fold higher concentration than inner membranes. The mitochondrial content in neutral glycolipids was 0.6 nmol/mg protein. The long‐chain bases found in free ceramides were d18:1 sphingosine, d18:0 3‐ketosphinganine and t21:1 phytosphingosine in increasing order. In contrast, 3‐ketosphinganine was the only base of glucosylceramide and lactosylceramide of inner membranes, whereas d18:1 sphingosine was the major long‐chain base of glucosylceramide of outer membranes.