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The human PER1 gene is transcriptionally regulated by multiple signaling pathways
Author(s) -
Motzkus Dirk,
Maronde Erik,
Grunenberg Uwe,
Lee Cheng Chi,
Forssmann Wolf-Georg,
Albrecht Urs
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02315-2
Subject(s) - per1 , microbiology and biotechnology , reporter gene , protein kinase c , circadian clock , gene , signal transduction , endogeny , biology , regulation of gene expression , response element , clock , gene expression , promoter , genetics , biochemistry
The mammalian period ( Per ) genes are components of the circadian clock and appear to be regulated via an autoregulatory feedback loop. Here we show that the human PER1 ( hPER1 ) gene is synergistically activated by protein kinases A and C (PKA, PKC) and cAMP responsive element binding protein. Activators and inhibitors of PKA as well as PKC modulate endogenous hPER1 expression and hPER1 promoter‐driven reporter gene activity in a dose‐dependent manner. Our results suggest that the hPER1 promoter acts as a sensor for multiple signaling molecules thereby integrating different physiological parameters. This regulation of hPER1 appears to be significant for rapid adaptation to changing environmental conditions.