Premium
Enhanced release of soluble urokinase receptor by endothelial cells in contact with peripheral blood cells
Author(s) -
Mustjoki Satu,
Sidenius Nicolai,
Vaheri Antti
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02312-7
Subject(s) - urokinase receptor , supar , microbiology and biotechnology , endothelial stem cell , receptor , peripheral blood mononuclear cell , chemistry , chemotaxis , cell , cell adhesion , biology , biochemistry , in vitro
The urokinase receptor (uPAR) on the cell surface plays an important role in extracellular proteolysis, cell migration and adhesion. Soluble uPAR (suPAR) has been recently discovered in plasma, but its origin is unclear. Our results now demonstrate that both unstimulated blood mononuclear and endothelial cells can release suPAR and that the release is enhanced when either mononuclear cells or thrombocytes are cultured together with endothelial cells. Co‐culture without cell–cell contacts fails to enhance suPAR release. We also found suPAR fragments, known to be potent inducers of chemotaxis, in co‐culture growth medium samples. Taken together, our results suggest that normal plasma suPAR can be produced by endothelial and mononuclear cells and that suPAR release in cell–cell contacts may have a regulatory role in cell adhesion.