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The biochemical profile of rat testicular tissue as measured by magic angle spinning 1 H NMR spectroscopy
Author(s) -
Griffin J.L.,
Troke J.,
Walker L.A.,
Shore R.F.,
Lindon J.C.,
Nicholson J.K.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02307-3
Subject(s) - nuclear magnetic resonance spectroscopy , magic angle spinning , magic angle , chemistry , spectroscopy , extracellular , sperm , creatine , nuclear magnetic resonance , biology , biochemistry , stereochemistry , physics , botany , quantum mechanics
The testis is the principal organ of male fertility, responsible for the production of spermatozoa and their maturation into sperm. However, the underlying biochemistry of the testis is relatively understudied. The fluidic and homogeneous nature of the testis makes it an ideal organ for high resolution magic angle spinning (MAS) 1 H NMR spectroscopy. In this study we have catalogued the low molecular weight metabolites. The testis contains large amounts of creatine, of which a substantial proportion was shown to be extracellular using bipolar gradients to measure apparent diffusion coefficients. The tissue also contained relatively high amounts of uridine.