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Epigenes: design and construction of new hereditary units
Author(s) -
Tchuraev R.N,
Stupak I.V,
Tropynina T.S,
Stupak E.E
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02300-0
Subject(s) - lac repressor , plasmid , genetics , inheritance (genetic algorithm) , gene , encoding (memory) , recombinant dna , lac operon , biology , physics , neuroscience
A plasmid digene construction designed before [Tchuraev, R.N. (1982) J. Gen. Biol. 43, 79–87] has been realised, including feedback by repressing proteins with given trigger regime of gene functioning. Experimental tests of the expected epigene properties of the obtained pECPI recombinant plasmid involving lacI and cI 857 regulatory genes have shown a phenomenon of steady inheritance of two alternative epigenotypes lacI 1 cI 0 and lacI 0 cI 1 , as well as an external toggle switch through metabolitic and temperature signals from one inherited functional state of the cyclic digene system into another. Thus, we have constructed a hereditary unit of a specific kind, namely, a two‐component stationary epigene with preset properties.