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Genomic Exploration of the Hemiascomycetous Yeasts: 10. Kluyveromyces thermotolerans
Author(s) -
Malpertuy Alain,
Llorente Bertrand,
Blandin Gaëlle,
Artiguenave François,
Wincker Patrick,
Dujon Bernard
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02281-x
Subject(s) - kluyveromyces , gene , saccharomyces cerevisiae , biology , genome , genetics
A genomic exploration of Kluyveromyces thermotolerans was performed by random sequence tag (RST) analysis. We sequenced 2653 RSTs corresponding to inserts sequenced from both ends. We performed a systematic comparison with a complete set of proteins from Saccharomyces cerevisiae , other completely sequenced genomes and SwissProt. We identified six mitochondrial genes and 1358–1496 nuclear genes by comparison with S. cerevisiae . In addition, 25 genes were identified by comparison with other organisms. This corresponds to about 24% of the estimated gene content of this organism. A lower level of conservation is observed with orthologues to genes of S. cerevisiae previously classified as orphans. Gene order was found to be conserved between S. cerevisiae and K. thermotolerans in 56.5% of studied cases.