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Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii
Author(s) -
Casaregola Serge,
Lépingle Andrée,
Bon Elisabeth,
Neuvéglise Cécile,
Nguyen Huu-Vang,
Artiguenave François,
Wincker Patrick,
Gaillardin Claude
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02278-x
Subject(s) - kluyveromyces lactis , saccharomyces cerevisiae , genetics , schizosaccharomyces pombe , transposable element , plasmid , biology , homology (biology) , gene , genome , genbank , genomic dna , open reading frame , peptide sequence
The genome of Saccharomyces servazzii was analyzed with 2570 random sequence tags totalling 2.3 Mb. BLASTX comparisons revealed a minimum of 1420 putative open reading frames with significant homology to Saccharomyces cerevisiae (58% aa identity on average), two with Schizosaccharomyces pombe and one with a human protein, confirming that S. servazzii is closely related to S. cerevisiae . About 25% of the S. servazzii genes were identified, assuming that the gene complement is identical in both yeasts. S. servazzii carries very few transposable elements related to Ty elements in S. cerevisiae . Most of the mitochondrial genes were identified in eight contigs altogether spanning 25 kb for a predicted size of 29 kb. A significant match with the Kluyveromyces lactis linear DNA plasmid pGKL‐1 encoded RF4 killer protein suggests that a related plasmid exists in S. servazzii . The sequences have been deposited with EMBL under the accession numbers AL402279–AL404848.