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Genomic Exploration of the Hemiascomycetous Yeasts: 2. Data generation and processing
Author(s) -
Artiguenave François,
Wincker Patrick,
Brottier Phillippe,
Duprat Simone,
Jovelin Fabien,
Scarpelli Claude,
Verdier Jean,
Vico Virginie,
Weissenbach Jean,
Saurin William
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02273-0
Subject(s) - dna sequencing , computational biology , computer science , dna sequencer , yeast , sequence (biology) , plasmid , biology , dna , genetics
The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra‐red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length.