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Effects of homocysteine on the binding of extracellular‐superoxide dismutase to the endothelial cell surface
Author(s) -
Yamamoto Masayuki,
Hara Hirokazu,
Adachi Tetsuo
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02260-2
Subject(s) - superoxide dismutase , chemistry , extracellular , homocysteine , plasma homocysteine , biochemistry , superoxide , microbiology and biotechnology , biophysics , biology , enzyme
Homocysteine is known to be a risk factor for several vascular diseases. Previously, we found a significant association between plasma homocysteine and plasma extracellular‐superoxide dismutase (EC‐SOD) levels. The binding of EC‐SOD to human and bovine aortic endothelial cell cultures showed significant decreases after incubation with 10 μM homocysteine, whereas the expression of EC‐SOD in fibroblast cell cultures was inhibited with a high concentration (1 mM) of homocysteine. Furthermore, binding of EC‐SOD to heparin immobilized on plates was decreased with homocysteine. These observations suggested that homocysteine decreases the binding of EC‐SOD to vascular endothelial cell surfaces by degradation of endothelial heparan sulfate proteoglycan, which results in a loss of the ability to protect endothelial cell surfaces from oxidative stress.