Premium
PA‐FABP, a novel marker of human epidermal transit amplifying cells revealed by 2D protein gel electrophoresis and cDNA array hybridisation
Author(s) -
O'Shaughnessy Ryan F.L.,
Seery John P.,
Celis Julio E.,
Frischauf Anna-Maria,
Watt Fiona M.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02252-3
Subject(s) - proteomics , biology , microbiology and biotechnology , population , stem cell , keratin , epidermis (zoology) , downregulation and upregulation , complementary dna , stem cell marker , blot , biochemistry , gene , genetics , demography , anatomy , sociology
Human epidermal stem cells express higher levels of β1 integrins than their more differentiated daughters, transit amplifying cells. In a search for additional stem and transit cell markers we used proteomics and differential cDNA hybridisation to compare keratinocytes fractionated on the basis of β1 integrin expression. There were remarkably few differences between the two populations and none of the RNAs differed in abundance by more than 2‐fold. Nevertheless, proteomics revealed upregulated expression of epidermal fatty acid binding protein (PA‐FABP, also known as E‐FABP), Annexin II and two keratin related proteins in the transit population. An unknown high molecular mass protein was upregulated in the stem cell population. The upregulation of PA‐FABP was confirmed by Northern blotting and conventional and whole mount labelling of human epidermis. We conclude that PA‐FABP is a novel marker of epidermal transit amplifying cells.