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Characterization of two distinct DP‐related genes from Arabidopsis thaliana 1
Author(s) -
Magyar Zoltán,
Atanassova Ana,
De Veylder Lieven,
Rombauts Stéphane,
Inzé Dirk
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02238-9
Subject(s) - arabidopsis , arabidopsis thaliana , gene , biology , phylogenetic tree , genetics , e2f , microbiology and biotechnology , gene expression , mutant
E2F/DP complexes play a pivotal role in the regulation of the G1/S transition in animals. Recently, plant E2F homologs have been cloned, but DP‐related sequences have not been identified so far. Here we report that Arabidopsis thaliana contains at least two different DP‐related genes, AtDPa and AtDPb . They exhibit an overall domain organization similar to that of their animal counterparts, although phylogenetic analysis demonstrated that they form a separate subgroup. AtDPs efficiently heterodimerize in vitro with the Arabidopsis E2F‐related proteins, AtE2Fa and AtE2Fb through their dimerization domains. AtDPa and AtE2Fa are predominantly produced in actively dividing cells with highest transcript levels in early S phase cells.