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Antioxidant actions of ovothiol‐derived 4‐mercaptoimidazoles: glutathione peroxidase activity and protection against peroxynitrite‐induced damage
Author(s) -
Bailly Fabrice,
Zoete Vincent,
Vamecq Joseph,
Catteau Jean-Pierre,
Bernier Jean-Luc
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02234-1
Subject(s) - ebselen , peroxynitrite , chemistry , glutathione , antioxidant , glutathione peroxidase , thiol , biochemistry , peroxidase , pharmacology , superoxide , enzyme , biology
4‐Mercaptoimidazoles derived from the naturally occurring antioxidants, ovothiols, were tested for their glutathione peroxidase‐like (GSH Px‐like) activity and protection against peroxynitrite‐induced damage. All the thiol compounds displayed similar significant GSH Px‐like activities, which are however weaker than that of the reference compound, ebselen. The inhibitions of the peroxynitrite‐dependent oxidation of Evans blue dye and dihydrorhodamine 123 showed that the thiol compounds substituted on position 5 of the imidazole ring were nearly as effective as ebselen while the C‐2 substituted ones were less effective. Both assays corroborate the large superiority of mercaptoimidazoles over glutathione as inhibitors of peroxynitrite‐dependent oxidation.