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Leukemia‐associated Rho guanine nucleotide exchange factor (LARG) links heterotrimeric G proteins of the G 12 family to Rho
Author(s) -
Fukuhara Shigetomo,
Chikumi Hiroki,
Gutkind J.Silvio
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02224-9
Subject(s) - heterotrimeric g protein , pleckstrin homology domain , guanine nucleotide exchange factor , biology , microbiology and biotechnology , signal transduction , signal transducing adaptor protein , genetics , g protein
A putative guanine nucleotide exchange factor (GEF), termed leukemia‐associated RhoGEF (LARG), was recently identified upon fusion to the coding sequence of the MLL gene in acute myeloid leukemia. Although the function of LARG is still unknown, it exhibits a number of structural domains suggestive of a role in signal transduction, including a PDZ domain, a LH/RGS domain, and a Dbl homology/pleckstrin homology domain. Here, we show that LARG can activate Rho in vivo. Furthermore, we present evidence that LARG is an integral component of a novel biochemical route whereby G protein‐coupled receptors (GPCRs) and heterotrimeric G proteins of the Gα 12 family stimulate Rho‐dependent signaling pathways.