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Adrenaline potentiates PI 3‐kinase in platelets stimulated with thrombin and SFRLLN: role of secreted ADP
Author(s) -
Selheim Frode,
Frøyset Ann Kristin,
Strand Ingrid,
Vassbotn Flemming S,
Holmsen Holm
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02188-8
Subject(s) - thrombin , platelet , thrombin receptor , chemistry , stimulation , receptor , pi , biochemistry , microbiology and biotechnology , endocrinology , medicine , biology
Adrenaline significantly potentiated late thrombin‐ and SFRLLN‐induced PtdIns(3,4)P 2 production. Furthermore, the potentiating effect of adrenaline on thrombin‐induced PtdIns(3,4)P 2 production was independent on secreted ADP, whereas, the effect of adrenaline on SFRLLN‐induced PtdIns(3,4)P 2 production was completely dependent of secreted ADP. However, the ADP‐dependent accumulation of PtdIns(3,4)P 2 was not required for irreversible platelet aggregation induced by SFRLLN in the presence of adrenaline. It is concluded that adrenaline can replace secreted ADP to potentiate PtdIns(3,4)P 2 production in thrombin‐stimulated but not in SFRLLN‐stimulated platelets, thus demonstrating a qualitative difference between platelet stimulation by thrombin and the thrombin receptor activating peptide SFRLLN.