Premium
Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides 1
Author(s) -
Aussel Laurent,
Chaby Richard,
Le Blay Karine,
Kelly John,
Thibault Pierre,
Perry Malcolm B.,
Caroff Martine
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02181-5
Subject(s) - chemistry , bordetella bronchiseptica , mass spectrometry , bordetella , serology , electrospray ionization , polysaccharide , chromatography , matrix assisted laser desorption/ionization , polyclonal antibodies , chemical structure , bordetella pertussis , microbiology and biotechnology , bacteria , desorption , biochemistry , antibody , biology , organic chemistry , genetics , adsorption , immunology
Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas‐liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix‐assisted laser desorption/ionization and electrospray. The following structure for the O‐chain‐free LPS was deduced from the experimental results: Mass spectrometry and serology revealed that the O‐chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species.