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The open reading frame of the Na + ‐dependent glutamate transporter GLAST‐1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain
Author(s) -
Huggett Jim,
Vaughan-Thomas Anne,
Mason Deborah
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02175-x
Subject(s) - open reading frame , exon , microbiology and biotechnology , messenger rna , biology , rna splicing , alternative splicing , cerebellum , chemistry , gene , peptide sequence , biochemistry , rna , endocrinology
Previously we have reported expression of an mRNA with homology to the Na + ‐dependent glutamate transporter, GLAST‐1, in bone. Here we demonstrate that the complete open reading frame of GLAST‐1 mRNA and corresponding 69 kDa protein are expressed in rat bone in vivo. We have also discovered a novel splice variant (GLAST‐1a), lacking exon 3, expressed in rat bone and brain. A 55 kDa protein detected by anti‐GLAST antibody in rat cerebellum corresponds to the molecular weight of unglycosylated GLAST‐1a. This has led us to propose that GLAST‐1a has an opposite orientation in the cell membrane to GLAST‐1.