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Enhanced epinephrine‐induced platelet aggregation in individuals carrying the G protein β3 subunit 825T allele
Author(s) -
Naber Christoph,
Hermann Burkhard Lorenz,
Vietzke Delia,
Altmann Christoph,
Haude Michael,
Mann Klaus,
Rosskopf Dieter,
Siffert Winfried
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02158-x
Subject(s) - heterotrimeric g protein , adenylyl cyclase , pertussis toxin , g protein , epinephrine , gs alpha subunit , gi alpha subunit , allele , platelet , stimulation , protein subunit , chemistry , biology , endocrinology , medicine , microbiology and biotechnology , signal transduction , gene , biochemistry
The 825T allele of a common C825T polymorphism in the gene encoding the β3 subunit of heterotrimeric G proteins is associated with enhanced activation of pertussis toxin (PTX)‐sensitive G proteins. We investigated responses of human platelets upon stimulation with epinephrine, which activates PTX‐sensitive G proteins, and with agonists which activate additionally, or exclusively PTX‐insensitive pathways. Slopes and maximum of the secondary aggregation were significantly enhanced in platelets from 825T allele carriers after epinephrine, and after combined epinephrine/ADP. This effect was more pronounced after inhibition of the cyclooxygenase‐2 pathway by acetylsalicylic acid. This phenomenon appeared independent of platelet secretion, or inhibition of the adenylyl cyclase.