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A tetanus toxin sensitive protein other than VAMP 2 is required for exocytosis in the pancreatic acinar cell
Author(s) -
Padfield Philip J
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02126-8
Subject(s) - exocytosis , toxin , secretion , chemistry , neurotoxin , botulinum toxin , adp ribosylation , acinar cell , biochemistry , microbiology and biotechnology , biology , pancreas , enzyme , neuroscience , nad+ kinase
The neurotoxin sensitivity of regulated exocytosis in the pancreatic acinar cell was investigated using streptolysin‐O permeabilized pancreatic acini. Treatment of permeabilized acini with botulinum toxin B (BoNT/B) or botulinum toxin D (BoNT/D) had no detectable effect on Ca 2+ ‐dependent amylase secretion but did result in the complete cleavage of VAMP 2. In comparison, tetanus toxin (TeTx) treatment both significantly inhibited Ca 2+ ‐dependent amylase secretion and cleaved VAMP 2. These results indicate that regulated exocytosis in the pancreatic acinar cell requires a tetanus toxin sensitive protein(s) other than VAMP 2.