Premium
Electroporation‐mediated gene transfer in free‐swimming embryonic Xenopus laevis
Author(s) -
Eide Fernette F,
Eisenberg Staci R,
Sanders Timothy A
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02124-4
Subject(s) - xenopus , electroporation , transgenesis , biology , microbiology and biotechnology , embryonic stem cell , developmental biology , embryogenesis , zebrafish , embryo , gene expression , anatomy , gene , genetics , reproductive technology
Xenopus laevis are a rich resource for vertebrate embryology and cell biology. Transplantation and transgenesis have provided much information about the developmental mechanisms of embryogenesis and molecule function, however existing methods have faced limitations regarding either the precise localization of gene expression or flexibility in the timing of gene transfer. Here we have found that electroporation of tailbud (stage 29/30) embryos is a rapid and efficient method of combining cell‐specific expression with variation in temporal delivery. At the low voltages required for electroporation, embryos resumed normal swimming behavior and development. We conclude that electroporation has wide experimental application to Xenopus developmental and cell biology.