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Effect of genomic and subgenomic leader sequences of potato leafroll virus on gene expression
Author(s) -
Juszczuk Marek,
Pączkowska Elżbieta,
Sadowy Ewa,
Zagórski Włodzimierz,
Hulanicka Danuta M.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02122-0
Subject(s) - subgenomic mrna , gene , biology , translation (biology) , potato leafroll virus , sequence (biology) , enhancer , genetics , virology , virus , microbiology and biotechnology , gene expression , messenger rna , rna , plant virus
The effect of the genomic and subgenomic leader sequence of potato leafroll polerovirus on the efficiency of translation of the downstream located genes has been studied. The results obtained in vitro and in vivo indicate that neither leader sequence functions as translational enhancer, a generally important feature of leader sequences. Deletion analyses demonstrated that both leader sequences not only decrease translation of the downstream located genes but also alter the ratio of the synthesized proteins. A correlation between the in vitro and in vivo results can be established in the case of the subgenomic leader sequence.