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Two nuclear localization signals are required for nuclear translocation of nuclear factor 1‐A
Author(s) -
Imagawa Masayoshi,
Sakaue Reiko,
Tanabe Atsuhiro,
Osada Shigehiro,
Nishihara Tsutomu
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02119-0
Subject(s) - nuclear localization sequence , chromosomal translocation , nls , nuclear transport , nucleus , transcription factor , cell nucleus , cytoplasm , microbiology and biotechnology , subcellular localization , biology , gene isoform , nuclear protein , gene , chemistry , genetics
Nuclear factor 1 (NF1) proteins are encoded by at least four genes (NF1‐A, B, C, X). Although DNA‐binding and the transcription regulation domains of these proteins are well characterized, the nuclear localization signals (NLSs) are still unknown in all NF1s. We have identified two NLSs in NF1‐A, and both are required for full translocation to the nucleus, although one of them itself has a partial translocation ability. These two NLSs are conserved in all four NF1s. Interestingly, three isoforms of NF1‐A (NF1‐A1, A2, A4) have two NLSs and translocate completely to the nucleus. In contrast, NF1‐A3 lacks the second NLS and partially stays in the cytoplasm. Since NF1s construct homodimer and heterodimer, these findings indicate the differential regulations of the NF1 translocation.