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The MAT1 cyclin‐dependent kinase‐activating kinase (CAK) assembly/targeting factor interacts physically with the MCM7 DNA licensing factor
Author(s) -
Wang Yan,
Xu Fan,
Hall Frederick L.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02117-7
Subject(s) - minichromosome maintenance , microbiology and biotechnology , kinase , immunoprecipitation , two hybrid screening , biology , yeast , cyclin dependent kinase , dna , genetics , dna replication , gene , eukaryotic dna replication , cell cycle
MAT1 (ménage à trois1) functions as an assembly/targeting factor of CAK (cyclin‐dependent kinase‐activating kinase). In a search for MAT1‐interacting proteins using yeast two‐hybrid system, MCM7 (minichromosome maintenance 7), a member of a family of DNA licensing factors, was identified. The physical interaction between MAT1 and MCM7 was confirmed in vivo in yeast cells and verified with in vitro protein binding assays. Further studies showed the RING‐finger motif of MAT1 is not required for the interaction with MCM7, while the C‐terminal domain of MAT1 is indispensable. Immunoprecipitation of MCM7 in human osteosarcoma MG63 cells demonstrated that MCM7 associates with the CAK complex in vivo.