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The rat hepatoma cell line H4‐II‐E‐C3 expresses high activities of the high‐affinity glutamate transporter GLT‐1A
Author(s) -
Pollard Matthew,
McGivan John
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02114-1
Subject(s) - glutamate receptor , transporter , cloning (programming) , microbiology and biotechnology , cell culture , glutamate aspartate transporter , messenger rna , blot , biology , cell , excitatory amino acid transporter , chemistry , biochemistry , gene , receptor , genetics , computer science , programming language
The rate of uptake of aspartate into the rat hepatoma cell line H4‐II‐E‐C3 is very much higher than that exhibited by normal rat hepatocytes. Using an RT‐PCR‐based strategy, a glutamate transporter resembling mouse liver GLT‐1A has been cloned from H4‐II‐E‐C3 cells. Northern blotting confirmed that relatively high levels of mRNA for GLT‐1A are expressed in hepatoma cells compared with negligible levels in rat hepatocytes. To our knowledge, this is the first report of the cloning of a high‐affinity glutamate transporter from a transformed cell line and also the first demonstration of functional expression of GLT‐1 outside the central nervous system.