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Inactivation of a MAPK‐like protein kinase and activation of a MBP kinase in germinating barley embryos
Author(s) -
Testerink Christa,
Vennik Marco,
Kijne Jan W.,
Wang Mei,
Heimovaara-Dijkstra Sjoukje
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02112-8
Subject(s) - protein kinase a , cyclin dependent kinase 2 , mitogen activated protein kinase kinase , kinase , map2k7 , c raf , immunoprecipitation , embryo , myelin basic protein , mapk/erk pathway , cyclin dependent kinase 9 , microbiology and biotechnology , biology , mitogen activated protein kinase , biochemistry , chemistry , myelin , endocrinology , gene , central nervous system
We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti‐MAPK (mitogen‐activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK‐like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti‐MAPK antibody and was induced only in germinating embryos, not in dormant embryos.