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Calcium channel β subunits differentially modulate recovery of the channel from inactivation
Author(s) -
Jeziorski Michael C,
Greenberg Robert M,
Anderson Peter A.V
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02098-6
Subject(s) - protein subunit , beta (programming language) , xenopus , calcium channel , gene isoform , g alpha subunit , biology , alpha (finance) , n type calcium channel , voltage dependent calcium channel , microbiology and biotechnology , chemistry , biophysics , calcium , biochemistry , gene , t type calcium channel , medicine , construct validity , nursing , organic chemistry , computer science , patient satisfaction , programming language
We examined the effects of calcium channel β subunits upon the recovery from inactivation of α 1 subunits expressed in Xenopus oocytes. Recovery of the current carried by the L‐type α 1 subunit ( cy Ca v 1) from the jellyfish Cyanea capillata was accelerated by coexpression of any β subunit, but the degree of potentiation differed according to which β isoform was coexpressed. The Cyanea β subunit was most effective, followed by the mammalian b 3 , b 4 , and β 2a subtypes. Recovery of the human Ca v 2.3 subunit was also modulated by β subunits, but was slowed instead. β 3 was the most potent subunit tested, followed by β 4 , then β 2a , which had virtually no effect. These results demonstrate that different β subunit isoforms can affect recovery of the channel to varying degrees, and provide an additional mechanism by which β subunits can differentially regulate α 1 subunits.