Premium
Genomic structure and promoter analysis of the rat Kir7.1 potassium channel gene ( Kcnj13 )
Author(s) -
Döring Frank,
Karschin Andreas
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02092-5
Subject(s) - tata box , promoter , biology , microbiology and biotechnology , gene , exon , choroid plexus , gene expression , 5' flanking region , caat box , reporter gene , repressor , potassium channel , genetics , central nervous system , neuroscience , biophysics
In the brain inwardly rectifying potassium channel Kir7.1 subunits are predominantly expressed in the choroid plexus and meninges. To investigate this tissue‐specific expression pattern, we characterized the genomic organization and the 5′ proximal promoter of the rat Kir7.1 gene ( Kcnj13 ). Starting from the major transcriptional initiation site, three exons in Kcnj13 give rise to the dominant ∼1.45 kb transcript in brain. Adjacent to the transcriptional start the minimal promoter which, uncommon for ion channels, contains a TATA‐ and CAAT‐box is controlled by AP‐1 factors and accounts for high gene expression levels. Luciferase reporter gene responses driven by the first 2.1 kb of the 5′ flanking region were similarly high in epithelial FRTL‐5 and neuronal N2A cells, suggesting that neuron‐specific repressor elements are located remote from the non‐selective minimal promoter.