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Nuclear localization of a hypoxia‐inducible novel non‐symbiotic hemoglobin in cultured alfalfa cells 1
Author(s) -
Seregélyes Csaba,
Mustárdy László,
Ayaydin Ferhan,
Sass László,
Kovács László,
Endre Gabriella,
Lukács Noémi,
Kovács Izabella,
Vass Imre,
Kiss György B.,
Horváth Gábor V.,
Dudits Dénes
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02049-4
Subject(s) - biology , complementary dna , gene , hemoglobin , microbiology and biotechnology , nucleus , cytoplasm , ploidy , cell nucleus , genetics , biochemistry
We have isolated a 483‐bp‐long full‐length cDNA clone encoding a non‐symbiotic hemoglobin called Mhb1 , the first one found in alfalfa. This non‐symbiotic hemoglobin is a single copy gene localized in linkage group 4 in diploid Medicago genome. The Mhb1 mRNA was found only in the roots of alfalfa plants. The Mhb1 gene was inducible by hypoxia and showed no induction by cold stress treatment. The Mhb1 transcript level increased at the G2/M boundary in a synchronized alfalfa cell suspension culture. The majority of Mhb1 protein was shown to be localized in the nucleus and smaller amounts were detected in the cytoplasm. A potential link to the nitric oxide signalling pathway is also discussed.