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Interaction of σ 70 with Escherichia coli RNA polymerase core enzyme studied by surface plasmon resonance
Author(s) -
Ferguson Anna L,
Hughes Amanda D,
Tufail Uzma,
Baumann Christoph G,
Scott David J,
Hoggett James G
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02028-7
Subject(s) - surface plasmon resonance , rna polymerase , escherichia coli , polymerase , dissociation constant , transcription (linguistics) , chemistry , microbiology and biotechnology , enzyme , dna , rna , biophysics , biology , gene , biochemistry , physics , receptor , linguistics , philosophy , quantum mechanics , nanoparticle
The interaction between the core form of bacterial RNA polymerases and σ factors is essential for specific promoter recognition, and for coordinating the expression of different sets of genes in response to varying cellular needs. The interaction between Escherichia coli core RNA polymerase and σ 70 has been investigated by surface plasmon resonance. The His‐tagged form of σ 70 factor was immobilised on a Ni 2+ –NTA chip for monitoring its interaction with core polymerase. The binding constant for the interaction was found to be 1.9×10 −7 M, and the dissociation rate constant for release of σ from core, in the absence of DNA or transcription, was 4×10 −3 s −1 , corresponding to a half‐life of about 200 s.