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Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis
Author(s) -
Peng Ying,
Kok K.H.,
Xu Ren-He,
Kwok K.H.H.,
Tay David,
Fung P.C.W.,
Kung Hsiang-fu,
Lin Marie C.M.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02019-6
Subject(s) - pronephros , xenopus , biology , kidney development , retinoic acid , microbiology and biotechnology , gene isoform , rna , embryonic stem cell , embryogenesis , embryo , gene , genetics
We cloned a major isoform of Xenopus homologue of cold inducible RNA binding protein (CIRP), XCIRP‐1. XCIRP‐1 was neither cold inducible nor essential for cell division during early embryonic development. Suppression of XCIRP‐1 dose dependently produced tailbuds with deformations of the brain and internal organs. The defects were XCIRP‐1 specific as they could be rescued by sense transcript. Suppression of XCIRP‐1 also disrupted the morphogenetic migration of the C3 blastomeres (lineaged to become the embryonic kidney, the pronephros). In animal cap explants, depletion of XCIRP‐1 inhibited activin/retinoic acid induced expressions of pronephros related Xlim‐1 and WT1 genes. These results suggest that XCIRP‐1 is required for the specification and morphogenetic lineage migration of the pronephros.