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CEO1, a new protein from Arabidopsis thaliana , protects yeast against oxidative damage 1
Author(s) -
Belles-Boix Enric,
Babiychuk Elena,
Van Montagu Marc,
Inzé Dirk,
Kushnir Sergei
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02016-0
Subject(s) - yap1 , yeast , arabidopsis , saccharomyces cerevisiae , arabidopsis thaliana , oxidative stress , biology , mutant , biochemistry , transcription factor , gene , complementary dna , dna damage , oxidative phosphorylation , microbiology and biotechnology , dna
The Saccharomyces cerevisiae strain WYT, deficient in the YAP1 transcription factor, was used in a molecular screen to identify genes from Arabidopsis thaliana that could overcome the oxidative stress‐sensitive phenotype of these yeast cells. A cDNA named CEO1 increased the tolerance to oxidative damage caused by tert ‐butylhydroperoxide of both the Yap1 − mutant and the wild‐type yeast. Additionally, in Yap1 − yeast, CEO1 also induced cross‐tolerance to oxidative damage caused by hydrogen peroxide and diamide. CEO1 was assigned as being part of a small gene family that, until now, is exclusively restricted to plants. In Arabidopsis , CEO1 was produced in all organs, especially in roots and stems. By using the yeast two‐hybrid system, proteins that specifically interact with CEO1 in yeast were identified, and putative DNA‐binding proteins were consistently recovered.