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Elevated expression of membrane type 1 metalloproteinase (MT1‐MMP) in reactive astrocytes following neurodegeneration in mouse central nervous system
Author(s) -
Rathke-Hartlieb Silvia,
Budde Petra,
Ewert Stefan,
Schlomann Uwe,
Staege Martin Sebastian,
Jockusch Harald,
Bartsch Jörg W.,
Frey Jürgen
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)02011-1
Subject(s) - astrogliosis , neurodegeneration , matrix metalloproteinase , astrocyte , downregulation and upregulation , microbiology and biotechnology , extracellular matrix , glial scar , neuroglia , biology , chemistry , central nervous system , pathology , endocrinology , biochemistry , medicine , disease , gene
Reactive astrocytes occurring in response to neurodegeneration are thought to play an important role in neuronal regeneration by upregulating the expression of extracellular matrix (ECM) components as well as the ECM degrading metalloproteinases (MMPs). We examined the mRNA levels and cellular distribution of membrane type matrix metalloproteinase 1 (MT1‐MMP) and tissue inhibitors 1–4 of MMPs (TIMPs) in brain stem and spinal cord of wobbler (WR) mutant mice affected by progressive neurodegeneration and astrogliosis. MT1‐MMP, TIMP‐1 and TIMP‐3 mRNA levels were elevated, whereas TIMP‐2 and TIMP‐4 expression was not affected. MT1‐MMP was expressed in reactive astrocytes of WR. In primary astrocyte cultures, MT1‐MMP mRNA was upregulated by exogeneous tumor necrosis factor α. Increased plasma membrane and secreted MMP activities were found in primary WR astrocytes.